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jove.com
Video: Author Spotlight: Optimizing CFU Determination for Efficient Assessment of TB Vaccine Efficacy and Antigen Presentation Analysis
2.5K Views. Universidade Católica Portuguesa. CFU determination is necessary to measure the efficacy of the immune response against bacteria. However, it is very well throughput and becomes cumbersome and expensive when applied to pathogenic bacteria such as mycobacterium tuberculosis. This is a significant limitation when we need to test ...
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Jul 28, 2023
Colony-forming unit Counting Methods
ISOLATION METHODS ·QUANTIFICATION BY COLONY COUNTINGINTRODUCTION LABORATORY SIMULATIONUsing the count data and observational data you acquired, calculate the number of CFUs in the original sample.Number of CFUs = CFUsWhy is it necessary to dilute a sample in order to determine bacterial numbers?A. The bacterial density in the original sample may be too high for the formation and counting of isolated colonies.B. The dilution process encourages the growth of more and different types of organisms,
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