Video: Polymerase chain reaction (PCR) and reverse-transcriptase PCR (RT-PCR) - Video Explanation! | Osmosis | Osmosis
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13 reactions | COVID-19 is diagnosed using real-time reverse transcriptase polymerase chain reaction (RT-PCR). In this video from our COVID Mini: Diagnostics course, you'll take a super deep dive into the RT-PCR process for SARS-CoV-2; learning about how it works, how it can go wrong, and a super important value you should look for that may help you predict disease progress. Watch full video: https://bit.ly/2ATdOsy | Medmastery | Facebook
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Arrange the steps of diagnosing AIDS using the RT PCR method in the right order.I. DNA, engineered primers specific to HIV genes, and nucleotides along with buffers are incubated in a thermal cycler.II. Viral RNA is extracted from the patient’s plasma or blood sample.III. The PCR product is run on a gel to separate and identify the PCR amplified viral genes.IV. Reverse transcriptase is used to convert the RNA to cDNA.
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